Fig. 2

The invasive phenotypes of EOC cells are regulated by miR-509-3p. A and B Upper panel: A2780CP70 and OVCAR-8 cells were transfected with miR-509-3p mimics and miR-509-3p expression levels were measured using real-time reverse transcription-polymerase chain reaction (RT-PCR) in were evaluated. Lower panel: A2780CP70 and OVCAR-8 cells were transfected with miR-509-3p mimics for 48 h; representative images of cell invasion is shown. All data represent the mean ± SD of experimental triplicates; * P < 0.05, ** P < 0.01, *** P < 0.001, compared with the control. C and D Upper panel: A2780 and OVCAR-3 cells were transfected with a miR-509-3p inhibitor and miR-509-3p expression levels were measured using real-time RT-PCR. Lower panel: A2780 and OVCAR-3 cells were transfected with a miR-509-3p inhibitor for 48 h; representative images of cell invasion is shown. All data represent the mean ± SD of experimental triplicates; * P < 0.05, ** P < 0.01, compared with the control. E Proliferation of A2780CP70 and A2780 cells transfected with miR-509-3p mimics and the miR-509-3p inhibitor, respectively, for 48 h. All experiments were performed in triplicate; with the corresponding * P < 0.05, ** P < 0.01, compared with the control. F A2780CP70 and A2780 cells were transfected with miR-509-3p mimics and the miR-509-3p inhibitor, respectively, for 48 h. Following transfection, cells were treated with different concentrations of cisplatin for 48 h. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays were performed to confirm cell viability in all assays E & F. All experiments were performed in triplicate