Fig. 6

Disruption of LCK leads to inhibition ovarian tumor treated with Olaparib (A) Schematic model of animal study. CP70 LCK KO and LCK OE cells were injected intraperitoneally in NSG mice. After 12 days, PARP inhibitor Olaparib i.p. (50 mg/kg) was administered 5 days/week. B After three weeks of Olaparib treatment, tumor volume was detected by IVIS imaging. C Tumor growth kinetics in tumor bearing mice injected with LCK KO and LCK OE CP70 cells. Mice were treated with vehicle or Olaparib. D TUNEL assay to detect DNA fragmentation in tumor tissue sections. TUNEL positive cells were counted from five images and plotted (Supplemental file S13 A). E IHC staining of γH2AX of tumor sections from different groups. γH2AX positive cells were counted from five images and plotted in graph (Supplemental file S13 B). F CD31 expression (Indicator of microvessel density and growth) of tumor sections from different group of mice. Microvessel density was counted from five images and plotted in graph (Supplemental file S13 C). Images are representative of two tumors from each cohort. We quantified the staining from 5 fields from each mouse. Images were captured at 20X magnification. One way ANOVA analysis was performed with Tukey's multiple comparisons test to compare different groups (* p < 0.05, ** p < 0.01, *** p < 0.001). Each experiment was repeated at least three times