Fig. 5

miR-4644 was negatively regulated by circ_0008285. A Direct binding sites of miR-4644 in WT 3ʹ-UTR of circ_0008285 and MUT 3ʹ-UTR of circ_0008285 were designed. B WT circ_0008285 or MUT circ_0008285 recombinant plasmids and miR-4644 mimic or mimic nc were co-transfected and luciferase reporter assay was performed. C The expression level of circ_0008285 in KGN cells when transfected with three HUPCOS shRNAs. D miR-4644 levels were detected using RT-PCR in KGN cell knockdown of circ_0008285. E circ_0008285 levels were detected using RT-PCR in KGN cell with miR-4644 mimic. *P < 0.05, **P < 0.01, ****P < 0.001