Fig. 6

miR-4644 directly targeted downstream LDLR. A Downstream mRNAs expression levels after silencing circ_0008285. B Direct binding sites between miR-4644 and WT 3ʹ-UTR of LDLR are shown. MUT 3ʹ-UTR of LDLR was also designed. C Luciferase activity was tested in KGN cells transfected by WT LDLR or MUT LDLR recombinant plasmids and miR-4644 mimic or mimic nc. D LDLR mRNA expression was determined in miR-4644 mimic cells. E LDLR was measured using western blotting in miR-4644 mimic cells. LDLR mRNA expression (F) and protein level (G) were determined in KGN cells. *P < 0.05, **P < 0.01, ***P < 0.005