Fig. 11

Proliferation and Migration of EC Cells were Encouraged by IGF2. IGF2 mRNA expression was detected by qRT-PCR in hESC and EC cells (A). In Ishikawa and HEC-1 A cells transfected with si-IGF2 and IGF2-cDNA, IGF2 levels were measured by qRT-PCR (B). In transfected Ishikawa and HEC-1 A cells, CCK-8 represents the cell growth curve. Colony test on transfected Ishikawa and HEC-1 A cells (E). Transfected Ishikawa and HEC-1 A cells were tested for transwell invasion (F). *P < 0.05,**P < 0.01, ***P < 0.001