Fig. 1

Expression of c-fos and MALAT1 in in vivo mouse models and in vitro cell models of POI. A The targeting relationship of MALAT1 with transcription factor c-fos obtained from the hTFtarget database; B Body weight of mice measured 14 days after CTX and BUS induction. n = 12. * p < 0.05 vs. control mice; C The ovarian weight of mice measured 14 days after CTX and BUS induction. n = 12. * p < 0.05 vs. control mice; D ELISA for serum AMH, E2 and FSH levels in mice. * p < 0.05 vs. control mice; E H&E staining for ovarian tissue structure in mice and statistical results of the number of follicles in each ovary. n = 12. * p < 0.05 vs. control mice; F Cleaved-Caspase3 immunohistochemistry to detect apoptosis in ovaries of mice and statistical results of relative intensity of positive staining. n = 12. * p < 0.05 vs. control mice; G RT-qPCR to detect the expression of MALAT1 and c-fos in ovarian tissues of mice. * p < 0.05 vs. control mice; H Western blot to detect the protein expression of c-fos in ovarian tissues of mice and the statistical plot of the gray values of the protein bands. * p < 0.05 vs. control mice; I Cell viability detected by CCK-8. * p < 0.05 vs. NC cells; J Cell proliferation detected by EdU assay and statistical results of proliferation rate. * p < 0.05 vs. NC cells; K Cell apoptosis detected by flow cytometry and statistical results of apoptosis rate. * p < 0.05 vs. NC cells. L TUNEL staining for apoptosis detection and statistical results of apoptosis rate. * p < 0.05 vs. NC cells; M RT-qPCR for MALAT1 and c-fos expression. * p < 0.05 vs. NC cells; N Western blot for c-fos protein expression and the statistical plot of the gray values of the protein bands. * p < 0.05 vs. NC cells. Cell experiments were repeated three times