Fig. 2

Validation of the regulatory mechanism of c-fos on MALAT1. A The JASPAR-database-based online prediction analysis of c-fos binding sites in the promoter region of MALAT1; B RT-qPCR detection of the efficiency of interfering and overexpressing c-fos and the statistical plot of the gray values of the protein bands. * p < 0.05 vs. cells treated with oe-NC or sh-NC; C Western blot detection of the efficiency of interfering and overexpressing c-fos and the statistical plot of the gray values of the protein bands; D, E Dual-luciferase reporter assay to detect the effect of c-fos on MALAT1 viability. * p < 0.05 vs. cells treated with oe-NC; F ChIP assay to detect the binding of c-fos to MALAT1 promoter. * p < 0.05 vs. cells treated with oe-NC; G RT-qPCR to detect MALAT1 expression after knockdown or overexpression of c-fos. * p < 0.05 vs. cells treated with oe-NC or sh-NC. Cell experiments were repeated three times