Fig. 3

Effects of c-fos on apoptosis and proliferation of KGN cells through regulation of MALAT1 expression. A RT-qPCR to detect the efficiency of overexpressing and interfering MALAT1 expression. * p < 0.05 vs. cells treated with oe-NC or sh-NC. KGN cells were treated with sh-c-fos alone or combined with oe-MALAT1. B RT-qPCR or Western blot to detect the expression of MALAT1 and c-fos in cells and gray scale scanning of the protein bands; C CCK-8 assay to detect cell viability; D EdU assay to detect cell proliferation and statistical results of proliferation rate; E Flow cytometry to detect apoptosis and statistical results of apoptosis rate; F TUNEL staining to detect apoptosis and statistical results of apoptosis rate; G Western blot to detect the expression of proteins related to cell proliferation and apoptosis and statistical results of scale values of the protein bands. In Panels B-G, * p < 0.05 vs. cells treated with sh-NC + oe-NC; # p < 0.05 vs. cells treated with sh-c-fos + oe-NC. Cell experiments were repeated three times