Fig. 4

Regulation of miR-22-3p expression by MALAT1 as a ceRNA. A Heatmap of differential expression of the top 20 miRNAs in terms of expression change in the bPOI-related miRNA expression microarray GSE100238 in the GEO database, where the color scale from blue to red indicates low to high expression; B Box plot of miR-22-3p expression in bPOI samples and control samples (bPOI: n = 10, Control: n = 10); C The target binding site of MALAT1 with miR-22-3p observed by the StarBase database; D RT-qPCR to detect miR-22-3p expression in animal and cell models. n = 12, * p < 0.05 vs. control mice or NC cells; E RT-qPCR to detect the efficiency of overexpressing and interfering miR-22-3p. * p < 0.05 vs. inhibitor-NC group or mimic-NC group; F Dual-luciferase reporter assay to detect targeted binding of MALAT1 to miR-22-3p. * p < 0.05 vs. mimic-NC group; G RIP assay to detect binding of MALAT1 to miR-22-3p. * p < 0.05 vs. anti-IgG group; H RNA pull-down assay to detect MALAT1 binding to miR-22-3p. * p < 0.05 vs. Bio-NC group. Cell experiments were repeated three times