Fig. 5

Validation of the targeting and regulation relationship between miR-22-3p and STAT1. A The target binding site of STAT1 with miR-22-3p found through the StarBase database. The up arrows indicate the existence of the binding site in human and down arrows indicate that in mice; B Western blot detection of STAT1 protein expression and phosphorylation level in mice and cell models and statistical results of scale values of the protein bands. n = 12, * p < 0.05 vs. control mice or NC cells; C Dual-luciferase reporter assay to detect the target binding site of STAT1 to miR-22-3p. * p < 0.05 vs. mimic-NC group; D RT-qPCR to detect STAT1 expression in cells. * p < 0.05 vs. the inhibitor-NC group or mimic-NC group; E Western blot to detect STAT1 protein expression and phosphorylation level in cells and statistical results of scale values of the protein bands. * p < 0.05 vs. inhibitor-NC group or mimic-NC group. Cell experiments were repeated three times