Fig. 6

Effects of c-fos on proliferation and apoptosis of KGN cells through regulation of the MALAT1/miR-22-3p/STAT1 ceRNA network. A Western blot for STAT1 protein expression and STAT1 phosphorylation level in cells treated with sh-MALAT1 alone or combined with miR-22-3p inhibitor, and statistical results of scale values of the protein bands. * p < 0.05 vs. cells treated with sh-NC + inhibitor-NC; # p < 0.05 vs. cells treated with sh-MALAT1 + inhibitor-NC. Cells were then treated with sh-c-Fos alone or combined with oe-STAT1. B, C RT-qPCR and Western blot for c-fos, STAT1, MALAT1 and miR-22-3p expression and STAT1 phosphorylation level and statistical results of scale values of the protein bands; D CCK-8 assay for cell viability; E EdU assay for cell proliferation and statistical results of proliferation rate; F Flow cytometry for apoptosis and statistical results of apoptosis rate; G TUNEL staining for apoptosis and statistical results of apoptosis rate; H Western blot to detect the expression of proteins related to cell proliferation and apoptosis and statistical results of scale values of the protein bands. In Panels B-H * p < 0.05 vs. cells treated with sh-NC + oe-NC; # p < 0.05 vs. cells treated with sh-c-fos + oe-NC. Cell experiments were repeated three times