Fig. 7

Effects of knockdown of c-fos or overexpression of STAT1 on POI mice. Note: POI mice were treated with sh-c-Fos alone or combined with oe-STAT1. A Western blot detection of c-fos and STAT1 protein expression and STAT1 phosphorylation level in ovarian tissues of mice and statistical results of scale values of the protein bands; B RT-qPCR detection of MALAT1 and miR-22-3p expression in ovarian tissues of mice; C Detection of body weight of mice; D Detection of ovarian weight of mice; E ELISA of serum AMH, E2 and FSH levels in mice; F H&E staining of ovarian tissue structure in mice and statistical results of the number of follicles in each ovary; G Cleaved-Caspase3 immunohistochemistry of ovarian apoptosis in mice and statistical results of relative intensity of positive staining. In Panels A-G * p < 0.05 vs. POI mice treated with sh-NC + oe-NC; # p < 0.05 vs. POI mice treated with sh-c-fos + oe-NC. n = 12