Fig. 2

DHEA-induced PCOS mouse displayed abnormalities in metabolism. The mice were divided into two groups (OIL, DHEA). (A) The body weight of OIL, OIL + DK, DHEA, DHEA + DK treated mice. Number within the bar indicates the weight of mice. Two-tailed, P < 0.05,(n = 3 mice per group). (B,C) GTT assay. AUC indicates area under the curve of GTT (n = 3 mice per group). (D,E) ITT assay. AUC indicates area under the curve of ITT (n = 3 mice per group). B,C,D,E used two-tailed, P < 0.05.(F) Compared the OIL and DHEA groups, hismorphology and expression of protein Ucp1 in brown adipose. (G) Ucp1,PPARα,PGC1α,Cited1,Cox8b mRNA expression levels in the brown adipose were measured by qPCR (n = 3 per group), Two-tailed, *P < 0.05, **P < 0.01, ***P < 0.001,**** P < 0.0001