Fig. 4

DK restores the abnormal hormone levels and estrus cycle in the PCOS mice. The mice were divided into four groups (OIL, OIL + DK, DHEA, DHEA + DK). (A) DK could partly reverse abnormal estrous cycles in the PCOS mice compared with abnormal estrous cycles in the DHEA treated mice. D, diestrus; E, estrus; M, metestrus; P, proestrus. (B, D) DK can significantly reduce the LH level and LH/FSH ratio caused by PCOS. (C, E) The serum level of follicle stimulating hormone (FSH) and estrogen, respectively. All data were analyzed by one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01,***P < 0.001 n = 3 per group