Fig. 5

DK significantly improves the characterization of metabolic abnormalities in the PCOS mice. The mice were divided into four groups (OIL, OIL + DK, DHEA, DHEA + DK). (A, B, C, D) DK has a certain therapeutic effect on the abnormal glucose tolerance and insulin resistance caused by DHEA injection, respectively. * means the significant differences of glucose level of DHEA compared to OIL group in the A and C. # means the significant differences of glucose level of DHEA + DK compared to OIL group in the A. B and D indicate area under the curve of GTT and ITT, respectively. Data were analyzed by one-way ANOVA with Tukey’s post hoc test. P < 0.05 n = 4 per group. (E) DK significantly reversed the whitening of brown adipose and the decrease of Ucp1 protein. (F) Ucp1 mRNA expression levels in the brown adipose were measured by qPCR (n = 3  per group). Data were analyzed by one-way ANOVA with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001, #P < 0.05, ##P < 0.01