Fig. 1

Over-expressed LPA regulates follicular microenvironment in vivo and in vitro. A Enzyme-linked immunosorbent assay result of LPA in follicular fluid of matured and immature oocytes (n = 21). (B) Cell counting kit-8 assay results of KGNs treated with different concentrations of LPA for 24 h. The protein levels of Ki67, PCNA, p62, LC3 II/I, Bcl-2, and Bax were detected by Western Blotting under different concentrations of LPA treatment (C-E, cropped gels). n = 3 per group in (B)-(E). Data are presented as means ± SEM; ^*P < 0.05, ^**P < 0.01, ^***P < 0.0001