Fig. 2

LPA regulates through the PI3K-AKT-mTOR pathway. A The protein levels of PI3K-AKT-mTOR pathway were detected by Western Blotting (cropped gels) under 24 h exposure of 10 μM LPA. B Serum-starved KGNs were treated with 20 μM LY294002 (PI3K inhibitor) for 24 h, as well as 5 mM 3MA (autophagy inhibitor) for 24 h, before exposure to 10 μM LPA for 24 h. The protein levels of PI3K-AKT-mTOR pathway were detected by Western Blotting (cropped gels). n = 3 per group. Data are presented as means ± SEM; ^*P < 0.05, ^**P < 0.01, ^***P < 0.0001 vs. CON, ^#P < 0.05, ^##P < 0.01, ^###P < 0.0001 vs. LPA