Fig. 4

Knockdown of LPAR1 attenuates LPA-mediated PI3K-AKT-mTOR pathway activation. A KGNs were seeded and treated 10 μM LPA for 24 h. LPAR1, LPAR2, and LPAR3 mRNA expression was analyzed by qRT-PCR. B LPAR1 was knocked down by siRNA with the primer sequences list in Table 1. Serum-starved KGNs were treated with 10 μM Ki16425 (LPAR1/3 inhibitor) for 24 h, before exposure to 10 μM LPA for 24 h. Also, control siRNA and LPAR1 siRNA1 were transfected into KGNs. C LPAR1 mRNA expression was verified by real-time PCR. D The protein levels of PI3K-AKT-mTOR pathway were detected by Western Blotting (cropped gels). n = 3 per group. Data are presented as means ± SEM; ^*P < 0.05, ^**P < 0.01, ^***P < 0.0001 vs. CON, ^#P < 0.05, ^##P < 0.01, ^###P < 0.0001 vs. LPA