Fig. 5

Knockdown of LPAR1 attenuates LPA-mediated autophagy enhancement. Serum-starved KGNs were treated with 10 μM Ki16425 (LPAR1/3 inhibitor) for 24 h, before exposure to 10 μM LPA for 24 h. Also, control siRNA and LPAR1 siRNA were transfected into KGNs. The autophagy related protein levels (A) and apoptosis related protein levels (B) were detected by Western Blotting (cropped gels). Immunofluorescence staining of LC3 (C), and flow cytometry analysis (D) were also taken. n = 3 per group. Data are presented as means ± SEM; ^*P < 0.05, ^**P < 0.01, ^***P < 0.0001 vs. CON, ^#P < 0.05, ^##P < 0.01, ^###P < 0.0001 vs. LPA