Fig. 2

Subcellular location of RBAT1 and its interaction with miR-27b. Both Ishikawa and HEC-1B cells were used to prepare nuclear and cytoplasm fractions, followed by RT-PCR to detect RBAT1 (A). IntaRNA 2.0 was applied to predict the direct interaction between them (B). RNA-RNA pulldowm assay was applied to determine the direct interaction between them (C). Dual luciferase reporter assay was performed by transfecting RBAT1 and miRNA NC or RBAT1 and miR-27b into cells. **, p < 0.01