Fig. 4

The role of RBAT1 and miR-27b in the viability of Ishikawa and HEC-1B cells. The viability of Ishikawa and HEC-1B cells under carboplatin and paclitaxel treatment was analyzed with MTT assay (A). Cell cycle distribution and apoptosis of Ishikawa and HEC-1B cells under carboplatin and paclitaxel treatment were measured by Flow Cytometry (B and C). Multidrug-resistant-related protein (P-gp, MRP1, and BCRP) expression levels were measured using RT-qPCR in Ishikawa and HEC-1B cells under carboplatin and paclitaxel treatment (D). The effect of RBAT1 and miR-27b knockdown on the viability (E), apoptosis (F), and cycle (G) of Ishikawa and HEC-1B cells with carboplatin and paclitaxel treatment. **, p < 0.01