Fig. 8From: Identification of transcriptome characteristics of granulosa cells and the possible role of UBE2C in the pathogenesis of premature ovarian insufficiencyThe expression of p21 was regulated by UBE2C in human granulosa cells. A qRT-PCR showed the mRNA expression of p21 in hGCs was upregulated in bPOI patients (n = 12 in each group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Mann–Whitney U-test). B The expression of p21, caspase-3, cleaved caspase-3 and Bax after knockdown of UBE2C were measured by Western blotting. C The expression of UBE2C and p21 after overexpression of UBE2C were measured by Western blotting. Relative expression of p21 and Bax were normalized to that of the β-actin and relative expression of cleaved caspase-3 were normalized to that of caspase-3. The expression of proteins was presented as the means ± SEM from three repeated experiments (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, two-tailed Student’s t-test). UBE2C: ubiquitin conjugating; SEM: standard error of meanBack to article page