Table 2 Sequences and parameters of primer used for qPCR
From: Cross talk of tumor protein D52 (TPD52) with KLF9, PKCε, and MicroRNA 223 in ovarian cancer
Name | Sequence | GC content % | Annealing Temperature |
|---|---|---|---|
KLF 9 Forward | 5′-TGGCTGTGGGAAAGTCTATGG-3′ | 52.4 | 60 °C |
KLF 9 Reverse | 5’-CTCGTCTGAGCGGGAGAACT-3 | 60 | 60 °C |
TPD52 Forward | 5-′GCTGCTTTTTCGTCTGTTGGCT-3′ | 50 | 60 °C |
TPD52 Reverse | 3′TCAAATGATTTAAAAGTTGGGGAGTT | 30 | 60 °C |
miR223 Forward | 5′-AGCCGTGTCAGTTTGTCAAAT-3′ | 42.9 | 60 °C |
miR-223Reverse | 5′-GTGCAGGGTCCGAGG TC-3′ | 70.6 | 60 °C |
PKCε Forward | 5′-AGCCTCGTTCACGGTTCT-3′ | 55.6 | 60 °C |
PKCε Reverse | 5′-TGTCCAGCCATCATCTCG-3′ | 55.6 | 60 °C |
beta-actin Forward | CACCATTGGCAATGAGCGGTTC | 54.5 | 60 °C |
beta-actin Reverse | AGGTCTTTGCGGATGTCCACGT | 54.5 | 60 °C |