Component | Supplier | Control medium | FLI medium |
---|---|---|---|
TCM199 | Sigma, M7528 | TCM199 | TCM199 |
Sodium pyruvate | Sigma, P4562 | 0.2 mM | 0.2 mM |
L-glutamin | Sigma, G8540 | 6.85 mM | 6.85 mM |
Cysteine | Sigma, C7352 | 0.57 mM | 0.57 mM |
Gentamycin | Roth, 0233 | 50 µg/ml | 50 µg/ml |
BSA | Sigma, A7030 | 1 mg/ml | 1 mg/ml |
PMSG | Prospec1, HOR-272 | 10 IU/ml | 10 IU/ml |
hCG | Prospec1, HOR-250 | 10 IU/ml | 10 IU/ml |
EGF | PeproTech2,AF-100-15 | 10 ng/ml | 10 ng/ml |
human LIF | Merck, LIF1005 | − | 2 µl/ml |
human IGF1 | PeproTech, AF-100-11 | − | 20 ng/ml |
human FGF2 | Sigma, F0291 | − | 40 ng/ml |