Fig. 3

The protective effect of VNE on antioxidative enzyme, superoxide dismutase (SOD) and catalase activities in ovarian tissue was investigated in LTZ-induced PCOS rats. The SOD and catalase activity in ovarian tissue were assessed using agarose gel electrophoresis followed by substrate-specific development of SOD and catalase bands (A). The lane distribution on the gel was as follows: Lane 1: control group; Lane 2: VNE group; Lane 3: LTZ-induced PCOS group; Lane 4: LTZ + VNE group. Ovarian tissue extracts containing equal amounts of proteins were subjected to electrophoresis on 12% or 8% native gel and 8% agarose gel, and the substrate-specific development of SOD and catalase bands was performed to evaluate the protective effect of VNE against LTZ-induced changes in SOD and catalase (A). Additionally, the spectrophotometric assay of SOD and catalase activity in ovarian tissue was conducted to further investigate the impact of VNE co-administration in LTZ-treated rats (B). Data represent as the Mean ± S.E.M. (n = 6), evaluated by ANOVA followed by the post hoc Tukey’s test. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. LTZ group