Mitigation of letrozole induced polycystic ovarian syndrome associated inflammatory response and endocrinal dysfunction by Vitex negundo seeds

Table 2 Effect of Vitex negundo extract on Serum hormones, steroidogenic enzymes and Estrogen receptor in letrozole induced PCOS rat

Parameters	Control	VNE	LTZ	LTZ + VNE
Estradiol (pg/ml)	587.65 ± 16.16	335.86 ± 19.34 ###	646.02 ± 34.93	366.00 ± 6.98 ###
Progesterone (ng/ml)	104.45 ± 4.84	116.84 ± 3.15 ###	65.07 ± 3.75 ***	117.34 ± 3.16 ###
Testosterone (ng/ml)	1.12 ± 0.215	1.05 ± 0.135 #	2.58 ± 0.522 *	1.16 ± 0.179 #
3β-HSD (ng/ml)	284.21 ± 13.40	280.94 ± 10.36 ###	396.08 ± 7.53 ***	375.86 ± 9.58
Aromatase (pg/ml)	1038.87 ± 34.55	1024.07 ± 33.56 ###	1522.62 ± 36.40 ***	1287.15 ± 48.06
ER-α (ng/ml)	3.731 ± 0.158	3.442 ± 0.170 ###	4.324 ± 0.159 *	3.125 ± 0.035 ###

Effects of Vitex nigundo on serum hormones (estradiol, progesterone and testosterone), steroidogenic enzymes (3β-HSD and aromatase), ER-α in rats with letrozole induced PCOS. Data presented as the Mean ± S.E.M. (n = 6), evaluated by ANOVA followed by the post hoc Tukey's test
*p<0.05, **p<0.01, ***p<0.001 vs. control; #p<0.05, ##p<0.01, ###p<0.001 vs. PCOS group. The inter and intra assay variation (CV) was <10 % for all parameters

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