Fig. 6

IHC analysis on PTEN, p-PTEN, p-AKT, FOXO3a, and p-FOXO3a in ovarian tissue of mice. Photomicrographs show hematoxylin and DAB-stained ovaries. (A1-E1) Control group. (A2-E2) POI group. (A3-E3) POI + UCMSCs group. (A4-E4) POI + LV-NC-UCMSCs group, (A5-E5) POI + LV-hsa-miR-21-UCMSCs group, (A6-E6) POI + LV-hsa-miR-21-inhibition-UCMSCs group. The statistical charts of the five kinds of cytokines expression in the six groups (A7-E7). Brown in cytoplasm indicates positive expression of the aimed protein. Blue represents cell nuclear staining. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. Control group or LV-NC-UCMSCs group, separately. Bar scale = 50 μm