Table 1 Sequences of the primers used in QRT-PCR.
Target gene | Primer | Nucleotide sequence |
|---|---|---|
h-Rnu6(U6) | F | 5’-TGCGGGTGCTCGCTTCGGCAGC-3’ |
R | 5’-CCAGTGCAGGGTCCGAGGTA-3’ | |
h-miR-21 | F | 5’-TAGCTTATCAGACTGATGTTG-3’ |
R | 5’-GCTGTCAACGATACGCTACGTAACG-3’ | |
mus-Rnu6(U6) | F R | 5’-CTCGCTTCGGCAGCACA-3’ 5’-AACGCTTCACGAATTTGCGT-3’ |
mus-miR-21 | F R | 5’-ACACTCCAGCTGGGTAGCTTATCAGACTGATG-3’ 5’-CTTAACGGCTGAGGTGCTGT-3’ |
mus-priMir21 | F R | 5’-GACATCGCATGGCTGTACCA-3’ 5’-CCATGATTCAACAGTCAACATCA-3’ |
mus-GAPDH | F | 5’-AGGTCGGTGAACGGATTTG-3’ |
R | 5’-TGTAGACCATGTAGTTGAGGTCA-3’ | |
mus-PTEN | F | 5’-TGGATTCGACTTAGACTTGACCT-3’ |
R | 5’-TGGCGGTGTCATAATGTCTCT-3’ | |
mus-FOXO3a | F | 5’-CTGGGGGAACCTGTCCTATG-3’ |
R | 5’-TCATTCTGAACGCGCATGAAG-3’ | |
mus-caspase3 | F | 5’-TGGTGATGAAGGGGTCATTTATG − 3’ |
R | 5’-TTCGGCTTTCCAGTCAGACTC-3’ |