Table 3 Primer details of hypermethylated genes for expression analysis by QRT-PCR
Gene | Primer Sequence (5’-3’) | Length | Tm | GC% | Product Length |
|---|---|---|---|---|---|
STK4 | Forward- ACGGTACAGCTGAGGAACCC Reverse-GCTGCCATAGGACCCTTCTCC | 20 21 | 61.54 62.26 | 60.00 61.90 | 123Â bp |
BMP2 | Forward- GGGACCCGCTGTCTTCTAGC Reverse-CGCAACTCGAACTCGCTCAG | 20 20 | 62.30 61.67 | 65.00 60.00 | 158Â bp |
CRKL | Forward- ACCCCGACTCACCTTGTGTG Reverse-GTGCAGAACTCAAGCTCGCC | 20 20 | 61.33 62.41 | 60.00 60.00 | 164Â bp |
PLXND1 | Forward- TGGGAAACTGATGGGGATCGT Reverse- AGCACGTAGGAGAAGCGGTC | 21 20 | 61.18 61.94 | 52.38 60.00 | 117Â bp |
POLR3B | Forward-GCAGTTTGCTTGGTGCAGGG Reverse-CGTCCATGCTGCTCACGAAG | 20 20 | 62.70 61.69 | 60.00 60.00 | 75Â bp |
GIGYF2 | Forward- CTGGGTCAGCCTTTATGCCAAG Reverse-GCTAGTCGCCAACCTCCATC | 22 20 | 61.53 60.53 | 54.55 60.00 | 158Â bp |