Fig. 2

TP63-truncated protein accelerated the cell apoptosis (A) TP63 exon 13 skipping resulted in the generation of a TP63-truncated protein p.S551Rfs*6. The plasmid expressing a p.S551* A TP63-truncated protein was constructed (TP63-mut). The empty vector (NC), TP63-WT, and TP63-mut were transfected into 293FT cells. The RT-qPCR results showed that the relative TP63 mRNA expression in TP63-mut was lower than that of the TP63-WT, and the ***p < 0.001. (B-C) The western immunoblotting assay showed that the expression of TP63 in TP63-mut was less than that of the TP63-WT. (D-E). The TP63-truncated protein accelerated apoptosis; Annexin-V was analyzed using Flowjo_v10.8.1 and GraphPad Prism. (F) The morphology of cells stained with Annexin-V, PI and DAPI was observed under a fluorescence microscope with a scale bar of 125 μm. The TP63-truncated protein accelerated apoptosis.The TP63-mut enhanced cell apoptosis