Fig. 3

The TP63-truncated protein activated additional expression of CLCA2, demonstrating that CLCA2 was the downstream target gene (A–C) The results of RNA-seq showed that there were 404 genes differentially expressed between TP63-WT and TP63-Mut, of which 265 genes were increased and 139 genes were decreased ((p < 0.05) and (|log2FC| > 1)). (D) GO analyses of TP63-WT and TP63-Mut genes with increased expression (Top: p < 0.05). (E–G) The mRNA level of CLCA2 (***p < 0.001) was higher in TP63-Mut than that in TP63-WT, and the western immunoblotting results showed greater expression of CLCA2 protein in TP63-Mut compared to that of TP63-WT. (H) The results of the luciferase assay showed that TP63-mut regulated the increased expression of the CLCA2 promoter (p < 0.001), indicating that CLCA2 was a downstream target gene of the TP63-truncated protein