Characterization of PLGA-nanoparticle (NP) containing curcumin (Nano-CUR) and its in vitro therapeutic efficacy. (A and B) Nano-CUR particles are an appropriate size of ~70 nm. Nano-CUR size was determined by (A) dynamic light scattering (DLS) and (B) transmission electron microscopy (TEM). (C) Nano-CUR formulation demonstrates sustained release of curcumin. Cumulative release of curcumin from PLGA NPs was determined by UV spectrophotometer at 450 nm over a period of 18 days. (D) Nano-CUR effectively inhibits the growth of cisplatin resistant ovarian cancer cells. A2780CP cells were treated with Nano-CUR (5-80 μM) or PLGA NPs without curcumin (NPs control) for 48 hrs. Cell proliferation was determined by MTS assay and normalized to control cells treated with vehicle (PBS). (E) A2780CP cells internalize PLGA-NPs. A2780CP cells were incubated with FITC-PLGA NPs for 6 hrs and analyzed by fluorescent microscopy. Original magnifications 400×. Inset image represents PLGA NPs no FITC. (F) Strategy used for antibody conjugation of PLGA-NP for targeted delivery of curcumin to ovarian cancer cells. (G) PLGA-NPs can be conjugated with anti-TAG-72 MAb (CC49). PLGA-NPs were incubated with anti-TAG-72 MAb. Nano-immunoconjugates were run on 10% SDS-PAGE, transferred to the PVDF membrane and were probed with an anti-mouse secondary antibody as indicated.