Ovarian stem cells: absence of evidence is not evidence of absence
© Bhartiya et al.; licensee BioMed Central Ltd. 2013
Received: 5 July 2013
Accepted: 15 September 2013
Published: 17 September 2013
Lei and Spradling in a recent study published in PNAS failed to detect ‘germline cysts’ by elegant studies using lineage tracing approach and thus concluded that adult mouse ovaries lack stem cells. They proposed that primordial follicle pool generated during fetal life is sufficient to sustain oogenesis and that there is no renewal of oocytes during adult life. Contrary to their results, we have reported presence of very small pluripotent, embryonic-like stem cells (VSELs), their immediate descendants (OGSCs) and germ cell ‘cysts’ or ‘nests’ (formed by rapid cell division and incomplete cytokinesis) in surface epithelial cell smears of adult sheep, monkey and human ovaries.
In the present study, ovaries were collected from adult mouse (treated with 5 IU pregnant mare serum gonadotropin, PMSG) and sheep (from slaughter house) and testis from mouse treated with busulphan (25 mg/Kg). Ovarian surface epithelial (OSE) cells and testicular smears were studied for the presence of cysts. Sheep OSE smears were also used to show cytoplasmic continuity amongst the cyst cells studied by immunolocalization and confocal microscopy of stem cells specific markers OCT-4 and SSEA-4.
Cysts were observed and confocal microscopy imaging confirmed cytoplasmic continuity amongst the cells comprising the cysts.
Cysts represent self-renewal and clonal expansion of stem cells with incomplete cytokinesis and are a hallmark feature of stem cells. We suggest the use of PMSG stimulated mouse ovaries and use of more primitive markers like OCT-4 or STELLA rather than MVH for lineage tracing studies to conclusively show presence of stem cells by lineage-tracing studies.
Sphere forming ability is considered a hallmark property of stem cells (cardiosphere, neurosphere, prostatesphere etc.) including cancer stem cells (mammosphere, melanospheres). The early embryo is indeed a sphere and embryonic stem cells also form sphere-shaped embryoid bodies. Similarly germ cell ‘nests’ or ‘cysts’ (germ cells that divide rapidly with incomplete cytokinesis) are well documented in the fetal ovaries [1, 2] and chains of clonally expanded stem cells exist in the testes . These structures possess multipotent properties and essentially represent self-renewal and clonal expansion of stem cells with incomplete cytokinesis.
Presence of mammalian ovarian stem cells is a highly debated area of reproductive biology since 2004, when Prof Jonathan Tilly from Harvard Medical School first challenged the long held central doctrine of fixed germ cell pool in females . Although compelling evidence has been provided by several groups in support of postnatal oogenesis, the subject has been challenged and negated by many including Lei & Spradling  who found no evidence of stem cells in mouse ovary using genetic recombination approach.
Evidence is emerging that various adult body organs possess two types of stem cells including dormant and active stem cells [5, 6]. Similarly two populations of stem cells including relatively quiescent very small embryonic-like stem cells (VSELs) and their actively dividing, immediate descendants ‘progenitors’ termed ovarian germ stem cells (OGSCs) have been reported by our group in mouse, rabbit, sheep, monkey and human ovary surface epithelium (OSE) . The stem cells at times are visualized as small clusters which are the germ cell ‘nests’ or ‘cysts’ representing self-renewal and clonal expansion. Twenty-one days long culture of OSE cells comprising these stem cells result in their spontaneous differentiation into oocyte-like structures . Further we have shown augmentation in process of neo-oogenesis and primordial follicle (PF) assembly in adult mice treated with PMSG . Ovarian cortical tissue cultures to study PF growth have also demonstrated stimulation of stem cells in presence of FSH and bFGF . Further stem cells in sheep OSE undergo clonal expansion and form cysts in response to FSH treatment and that this process is mediated through R3 isoform of FSH receptor . In this brief report we show that just like our earlier published data in rabbit, sheep, monkey and human ovaries, cysts are visualized in adult sheep and mouse ovaries and are more in number in PMSG treated mice.
Material and methods
For isolation of mouse OSE, normal and PMSG treated (5 IU subcutaneous) ovaries were subjected to enzymatic digestion using a protocol similar to one reported earlier by Symonds et al. . PMSG was injected during di-estrous phase of the estrus cycle and ovaries were collected after 24 hours. Briefly the ovaries were separated from the surrounding bursal tissue and carefully suspended in DMEM containing high glucose (Life Technologies). Each ovary was incubated at 37°C for 30 mins in 0.1 ml of the DMEM containing 0.5 mg/ml of Collagenase Type IV (Life Technologies). Tubes were briefly vortexed for 2 mins and the enzyme reaction was terminated by adding DMEM media supplemented with 15% fetal bovine serum (Life Technologies) and vortexed again for 2 mins after removal of ovaries. Smears were made from dissociated OSE cells on poly-l-lysine coated slides and fixed with 4% paraformaldehyde (PFA) followed by Haematoxylin and Eosin (H & E) staining. Sheep OSE cells were obtained by mechanical scraping of sheep ovaries  and smears fixed in 4% PFA and stained with H & E prior to viewing under an upright microscope (Nikon 90i, Japan). Busulphan treated mouse testis was used as a positive control to show the presence of stem cells and cyst-like structures. Briefly the mice were treated with 25 mg/Kg busulphan (Sigma) by intra-peritoneal route and after one month, the chemo-ablated mice testes were used for making smears. The smears were H & E stained and viewed under a microscope to capture representative images.
Germ cell markers OCT-4 and SSEA-4 were immunolocalized on sheep OSE smears and viewed under confocal microscope (LSM 510-META; ZEISS, Germany). Briefly, the cell smears were permeabilized with 0.3% Triton X-100 for 5 mins at room temperature for cytoplasmic marker (OCT-4) whereas this step was omitted for cell surface specific marker (SSEA-4). Non-specific epitopes were blocked by incubation with 10% serum of species specific to secondary antibody used in immuno-staining for one hour at room temperature. Cells were incubated overnight with respective primary antibody at 4°C and subsequently washed with washing buffer (DPBS containing 0.5% BSA and 0.1 mM EDTA) and later incubated with Alexafluor 488 (Molecular Probes, Invitrogen) labeled anti- rabbit IgG (1:1000) or anti-mouse IgG in wash buffer for 1 hour. First smears were washed with washing buffer several times, followed by counter staining for 30 seconds with propidium iodide (0.5 ug/ml) or DAPI (1.47 μM) (both from Sigma-Aldrich). Negative controls were also prepared by omission of primary antibody. Images of representative areas were captured by laser scanning confocal microscope at 63× magnification with further optical zoom using argon laser at λ = 488 nm, blue diode laser at λ = 405 and DPSS laser at λ = 561 nm for FITC, DAPI and PI staining channels respectively.
Results and Discussion
We have published several papers on gonadal VSELs [7–10, 12–14] and have shown that similar to the VSELs in the bone marrow and cord blood [15, 16], the VSELs in gonads are spherical cells, smaller than red blood cells, with high nucleo-cytoplasmic ratio, stain very dark with Haematoxylin, do not stain easily with DAPI, express pluripotent (OCT-4A, SSEA-4, Nanog, Sox-2, Rex), primordial germ cells (STELLA, FRAGILIS), VSELs (SCA-1, CD133) and germ cells (VASA, DAZL) specific markers. They are located in the ovary surface epithelium and in the basal epithelium of testicular seminiferous tubules. They are quiescent in nature and self-renew to give rise to the progenitors which divide rapidly with incomplete cytokinesis and occasionally appear as cysts and differentiate further and undergo meiosis to give rise to the gametes.
However, using mouse VASA homolog (MVH) as a marker and lineage labeling approach, Lei & Spradling  did not detect mitotically active germ cell progenitors and cysts, although mouse testis and fetal ovary used as positive controls showed the presence of cysts suggesting that their technique was perfect. Their method was based on detecting actively dividing cells, which would get lineage marked to express YFP through controlled use of tamoxifen, although the expression is not restricted only to the germ cells. As germ cell cysts are actively dividing, they are expected to get lineage marked and express YFP. But they used normal adult mouse ovaries for their study and based on our results and earlier reports [7, 8], they may have got positive results had they used mouse ovary after PMSG treatment. Neo-oogenesis in adult ovary is a very subtle process and thus has remained elusive for decades, compared to testes or fetal ovary where the order of magnitude of the same process is very high. A large number of sperm are produced in the testes and oocytes in fetal ovary compared to few oocytes per estrus cycle during adult life. At the dose of tamoxifen, which was used to control the number of cells expressing YFP by Lei and Spradling  it is quite possible that the subtle process of cyst formation in adult ovary remained undetected, even though it could be detected in testes and fetal ovary.
Interestingly, in the Lei and Spradling study the number of germ cell cysts that were lineage marked even in fetal ovary appear to be very few considering the high frequency of cysts present (please refer figure three of reference ). Also in adult testis the frequency of labeling was only 1.5% on an average (please refer Table one and Supplementary Figure one of reference ). Hence considering the very low frequency of labeling process, we think technical reasons might have contributed to failure of detection of germ cell cysts rather than absence of ovarian stem cells in adult ovary per se. We were even more surprised that the group expected stem cells to become functional after 4–14 days of busulphan treatment. It has been suggested that busulphan treatment destroys the somatic microenvironment of both testes and ovaries in addition to the germ cells [18, 19]. A better approach would have been to wait for a month after busulphan treatment for complete loss of germ cells/follicles and then stimulate the ovary to study stem cell activity. Spontaneous stem cell activity is not likely to occur immediately after chemotherapy.
Similarly, another group also demonstrated lack of mitotically active female germ cells using alternative lineage tracing approach, using MVH as a marker . We suggest technical limitations may exist in this study too and thus may have resulted in negative results. The important issue that needs to be resolved in this paper  is whether recombination by Cre occurs in ovarian stem cells in spite of them expressing MVH? In the original report that generated germ cell specific Vasa-Cre transgenic mice  it was reported that Vasa Cre activity was detectable only from e15-e18 in mouse testis even though Vasa expression in mice is reported as early as e11.5. The period of e15-e18 corresponds to meiosis and oocyte commitment and lack of mitosis in fetal ovaries. It was speculated that absence of remote or far away located regulatory elements might explain lack of earlier Cre activity. Another reason postulated was requirement of a threshold level of Cre protein to effectively induce recombination that was available only during the window period of e15-18 and thus enabled visual detection by Lac Z expression of Cre recombinase by X-gal method of detection and not earlier in spite of expression of MVH. This reason seems more likely because independent studies have shown that the levels of MVH increase by 2–2.5 fold in germ cells around e14.5 and e15.5 compared to e12.5 in both male and female mouse gonads [22, 23]. Also, in a previous report by Araki et al.  it has been shown that in ES cells the efficiency of Cre mediated recombination depends on strength of promoter activity under-which it is cloned suggesting the need for appropriate levels of Cre protein for effective recombination. Hence the need for a threshold level of Cre protein to effectively induce recombination and subsequent reporter expression becomes important in the context of conclusions made recently . The question that arises is whether the ovarian stem cells in adult mouse ovaries have strong enough MVH expression to induce sufficient Cre levels for recombination to occur? This seems unlikely since pre-meiotic mitotically active germ cells in fetal ovary do not exhibit Cre activity in spite of them expressing MVH (as these cells are observed prior to e15). Thus it is likely that even adult mitotically active ovarian stem cells and pre-meiotic germ cells may not exhibit Cre-induced recombination. Use of more primitive markers like OCT-4 or STELLA for lineage studies instead of MVH to conclusively determine the presence or absence of adult ovarian stem cells may be a better option. These markers are essential for oogenesis compared to MVH as knockout mice of these markers are known to be lethal or have led to complete loss of germ cells in females compared to MVH knockout, which does not affect female germ cells [25, 26].
Based on the results and discussions, it is concluded that the findings of the two studies published in PNAS [1, 20] should be interpreted with caution since absence of evidence is not necessarily the evidence of absence. Also we throw new light on stem cell biology in both ovary and testis.
- Lei L, Spradling AC: Female mice lack adult germ-line stem cells but sustain oogenesis using stable primordial follicles. Proc Natl Acad Sci U S A 2013, 110: 8585–8590. 10.1073/pnas.1306189110PubMed CentralPubMedView ArticleGoogle Scholar
- Pepling ME: Follicular assembly: mechanisms of action. Reproduction 2012, 143: 139–149. 10.1530/REP-11-0299PubMedView ArticleGoogle Scholar
- Lesch BJ, Page DC: Genetics of germ cell development. Nat Rev Genet 2012, 13: 781–794. 10.1038/nrg3294PubMedView ArticleGoogle Scholar
- Johnson J, Canning J, Kaneko T, Pru JK, Tilly JL: Germline stem cells and follicular renewal in the postnatal mammalian ovary. Nature 2004, 428: 145–150. 10.1038/nature02316PubMedView ArticleGoogle Scholar
- Li L, Clevers H: Coexistence of quiescent and active adult stem cells in mammals. Science 2010, 327: 542–545. 10.1126/science.1180794PubMed CentralPubMedView ArticleGoogle Scholar
- De Rosa L, De Luca M: Cell biology: dormant and restless skin stem cells. Nature 2012, 489: 215–217. 10.1038/489215aPubMedView ArticleGoogle Scholar
- Parte S, Bhartiya D, Telang J, Daithankar V, Salvi V, Zaveri K, Hinduja I: Detection, characterization, and spontaneous differentiation in vitro of very small embryonic-like putative stem cells in adult mammalian ovary. Stem Cells Dev 2011, 20: 1451–1464. 10.1089/scd.2010.0461PubMed CentralPubMedView ArticleGoogle Scholar
- Bhartiya D, Sriraman K, Gunjal P, Modak H: Gonadotropin treatment augments postnatal oogenesis and primordial follicle assembly in adult mouse ovaries? J Ovarian Res 2012, 5: 32. 10.1186/1757-2215-5-32PubMed CentralPubMedView ArticleGoogle Scholar
- Parte S, Bhartiya D, Manjramkar DD, Chauhan A, Joshi A: Stimulation of ovarian stem cells by follicle stimulating hormone and basic fibroblast growth factor during cortical tissue culture. J Ovarian Res 2013, 6: 20. 10.1186/1757-2215-6-20PubMed CentralPubMedView ArticleGoogle Scholar
- Patel H, Bhartiya D, Parte S, Gunjal P, Yedurkar S, Bhatt M: Follicle stimulating hormone modulates ovarian stem cells through alternately spliced receptor variant FSH-R3. J Ovarian Res 2013, 6: 52. 10.1186/1757-2215-6-52PubMed CentralPubMedView ArticleGoogle Scholar
- Symonds DA, Tomic D, Miller KP, Flaws JA: Methoxychlor induces proliferation of the mouse ovarian surface epithelium. Toxicol Sci 2005, 83: 355–362.PubMedView ArticleGoogle Scholar
- Bhartiya D, Unni S, Parte S, Anand S: Very small embryonic-like stem cells: implications in reproductive biology. Biomed Res Int 2013, 2013: 682326.PubMed CentralPubMedView ArticleGoogle Scholar
- Bhartiya D, Kasiviswanathan S, Unni SK, Pethe P, Dhabalia JV, Patwardhan S, Tongaonkar HB: Newer insights into premeiotic development of germ cells in adult human testis using Oct-4 as a stem cell marker. J Histochem Cytochem 2010, 58: 1093–1106. 10.1369/jhc.2010.956870PubMed CentralPubMedView ArticleGoogle Scholar
- Bhartiya D, Sriraman K, Parte S: Stem cell interaction with somatic niche may hold the key to fertility restoration in cancer patients. Obstet Gynecol Int 2012, 2012: 921082.PubMed CentralPubMedGoogle Scholar
- Ratajczak MZ, Suszynska M, Pedziwiatr D, Mierzejewska K, Greco NJ: Umbilical cord blood-derived very small embryonic like stem cells (VSELs) as a source of pluripotent stem cells for regenerative medicine. Pediatr Endocrinol Rev 2012, 9: 639–643.PubMedGoogle Scholar
- Bhartiya D, Shaikh A, Nagvenkar P, Kasiviswanathan S, Pethe P, Pawani H, Mohanty S, Rao SG, Zaveri K, Hinduja I: Very small embryonic-like stem cells with maximum regenerative potential get discarded during cord blood banking and bone marrow processing for autologous stem cell therapy. Stem Cells Dev 2012, 21: 1–6. 10.1089/scd.2011.0311PubMedView ArticleGoogle Scholar
- Zhang D, Fouad H, Zoma WD, Salama SA, Wentz MJ, Al-Hendy A: Expression of stem and germ cell markers within nonfollicular structures in adult mouse ovary. Reprod Sci 2008, 15: 139–146. 10.1177/1933719107310708PubMedView ArticleGoogle Scholar
- Oatley JM, Brinster RL: The germline stem cell niche unit in mammalian testes. Physiol Rev 2012, 92: 577–595. 10.1152/physrev.00025.2011PubMed CentralPubMedView ArticleGoogle Scholar
- Park MR, Choi YJ, Kwon DN, Park C, Bui HT, Gurunathan S, Cho SG, Song H, Seo HG, Min G, Kim JH: Intraovarian transplantation of primordial follicles fails to rescue chemotherapy injured ovaries. Sci Rep 2013, 3: 1384.PubMed CentralPubMedGoogle Scholar
- Zhang H, Zheng W, Shen Y, Adhikari D, Ueno H, Liu K: Experimental evidence showing that no mitotically active female germline progenitors exist in postnatal mouse ovaries. Proc Natl Acad Sci U S A 2012, 109: 12580–12585. 10.1073/pnas.1206600109PubMed CentralPubMedView ArticleGoogle Scholar
- Gallardo T, Shirley L, John GB, Castrillon DH: Generation of a germ cell- specific mouse transgenic Cre line, Vasa-Cre. Genesis 2007, 45: 413–417. 10.1002/dvg.20310PubMed CentralPubMedView ArticleGoogle Scholar
- Western PS, van den Bergen JA, Miles DC, Sinclair AH: Male fetal germ cell differentiation involves complex repression of the regulatory network controlling pluripotency. FASEB J 2010, 24: 3026–3035. 10.1096/fj.09-151555PubMedView ArticleGoogle Scholar
- van den Bergen JA, Miles DC, Sinclair AH, Western PS: Normalizing gene expression levels in mouse fetal germ cells. Biol Reprod 2009, 81: 362–370. 10.1095/biolreprod.109.076224PubMed CentralPubMedView ArticleGoogle Scholar
- Araki K, Imaizumi T, Okuyama K, Oike Y, Yamamura K: Efficiency of recombination by Cre transient expression in embryonic stem cells: comparison of various promoters. J Biochem 1997, 122: 977–982. 10.1093/oxfordjournals.jbchem.a021860PubMedView ArticleGoogle Scholar
- Tanaka SS, Toyooka Y, Akasu R, Katoh-Fukui Y, Nakahara Y, Suzuki R, Yokoyama M, Noce T: The mouse homolog of Drosophila vasa is required for the development of male germ cells. Genes Dev 2000, 14: 841–853.PubMed CentralPubMedGoogle Scholar
- Payer B, Saitou M, Barton SC, Thresher R, Dixon JP, Zahn D, Colledge WH, Carlton MB, Nakano T, Surani MA: Stella is a maternal effect gene required for normal early development in mice. Curr Biol 2003, 13: 2110–2117. 10.1016/j.cub.2003.11.026PubMedView ArticleGoogle Scholar
- Woods DC, Tilly JL: An evolutionary perspective on adult female germline stem cell function from flies to humans. Semin Reprod Med 2013, 31: 24–32. 10.1055/s-0032-1331794PubMedView ArticleGoogle Scholar
- Ratajczak MZ, Machalinski B, Wojakowski W, Ratajczak J, Kucia M: A hypothesis for an embryonic origin of pluripotent Oct-4(+) stem cells in adult bone marrow and other tissues. Leukemia 2007, 21: 860–867.PubMedGoogle Scholar
- Massasa E, Costa XS, Taylor HS: Failure of the stem cell niche rather than loss of oocyte stem cells in the aging ovary. Aging (Albany NY) 2010, 2: 1–2.Google Scholar
- Abbott A: Doubt cast over tiny stem cells. Nature 2013, 499: 390. 10.1038/499390aPubMedView ArticleGoogle Scholar
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