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Table 1 Ovary histomorphometry and VEGFA, Ki67 and CYP17 immunohistochemical data of the study groups

From: Metformin improves ovarian follicle dynamics by reducing theca cell proliferation and CYP-17 expression in an androgenized rat model

Variable

CG (n = 12)

GA (n = 12)

GAmet (n = 12)

Degenerating follicles

(No./slide)

4.11 ± 1.07

5.87 ± 1.87*

3.10 ± 1.10

Corpora lutea (No./10 fields)

8.26 ± 2.81**

3.06 ± 1.32

Non-antral follicles

(No./10 fields)

3.23 ± 1.41

5.98 ± 3.88

3.77 ± 2.80

Antral follicles (No./fields)

1.97 ± 1.87

2.89 ± 1.89

2.98 ± 1.95

Interstitial cells

(No./780-μm2 area)

162.29 ± 23.21

313.18 ± 26.38*

172.45 ± 37.85

Epithelial thickness (μm)

8.28 ± 0.10

8.59 ± 0.19

8.30 ± 0.15

VEGFA (index)

 Granulosa cells

2.6 ± 0.2

9.6 ± 0.3*

4.2 ± 0.6

 Theca interna cells

3.8 ± 0.2

10.7 ± 0.6*

5.1 ± 0.2

 Interstitial cells

2.6 ± 1.6

9.3 ± 0.3*

4.2 ± 0.2

Ki67 (%)

 Granulosa cells

32.0 ± 4.4

14.0 ± 3.3*

28.1 ± 2.5

 Theca interna cells

16.0 ± 2.2

34.0 ± 1.5*

18.0 ± 3.1

 Interstitial cells

1.2 ± 0.1

8.0 ± 0.3*

1.1 ± 0.4

CYP17 (%)

 Granulosa cells

1.1 ± 0.8

1.5 ± 1.1

1.2 ± 0.9

 Theca interna cells

5.5 ± 1.7

9.3 ± 1.2*

6.2 ± 0.7

 Interstitial cells

5.2 ± 0.8

7.4 ± 1.4*

4.2 ± 1.6

  1. Abbreviations: CG control group, GA androgenized group, GAmet androgenized + metformin group. The data for all the groups are presented as the means±standard deviations. The histological results were analyzed using analysis of variance (ANOVA) and Tukey’s test. The VEGFA, Ki67 and CYP17 immunohistochemical data were analyzed using the Kruskal-Wallis test and the Dunn test. *p < 0.01 compared with the other groups and **p < 0.05 compared with the GAmet group