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Table 1 Ovary histomorphometry and VEGFA, Ki67 and CYP17 immunohistochemical data of the study groups

From: Metformin improves ovarian follicle dynamics by reducing theca cell proliferation and CYP-17 expression in an androgenized rat model

Variable CG (n = 12) GA (n = 12) GAmet (n = 12)
Degenerating follicles
(No./slide)
4.11 ± 1.07 5.87 ± 1.87* 3.10 ± 1.10
Corpora lutea (No./10 fields) 8.26 ± 2.81** 3.06 ± 1.32
Non-antral follicles
(No./10 fields)
3.23 ± 1.41 5.98 ± 3.88 3.77 ± 2.80
Antral follicles (No./fields) 1.97 ± 1.87 2.89 ± 1.89 2.98 ± 1.95
Interstitial cells
(No./780-μm2 area)
162.29 ± 23.21 313.18 ± 26.38* 172.45 ± 37.85
Epithelial thickness (μm) 8.28 ± 0.10 8.59 ± 0.19 8.30 ± 0.15
VEGFA (index)
 Granulosa cells 2.6 ± 0.2 9.6 ± 0.3* 4.2 ± 0.6
 Theca interna cells 3.8 ± 0.2 10.7 ± 0.6* 5.1 ± 0.2
 Interstitial cells 2.6 ± 1.6 9.3 ± 0.3* 4.2 ± 0.2
Ki67 (%)
 Granulosa cells 32.0 ± 4.4 14.0 ± 3.3* 28.1 ± 2.5
 Theca interna cells 16.0 ± 2.2 34.0 ± 1.5* 18.0 ± 3.1
 Interstitial cells 1.2 ± 0.1 8.0 ± 0.3* 1.1 ± 0.4
CYP17 (%)
 Granulosa cells 1.1 ± 0.8 1.5 ± 1.1 1.2 ± 0.9
 Theca interna cells 5.5 ± 1.7 9.3 ± 1.2* 6.2 ± 0.7
 Interstitial cells 5.2 ± 0.8 7.4 ± 1.4* 4.2 ± 1.6
  1. Abbreviations: CG control group, GA androgenized group, GAmet androgenized + metformin group. The data for all the groups are presented as the means±standard deviations. The histological results were analyzed using analysis of variance (ANOVA) and Tukey’s test. The VEGFA, Ki67 and CYP17 immunohistochemical data were analyzed using the Kruskal-Wallis test and the Dunn test. *p < 0.01 compared with the other groups and **p < 0.05 compared with the GAmet group