Fig. 5
Effects of letrozole (LET) on gene expression of hormone receptors, Fshr, Cyp19a1, and Vegf. Isolated follicles were cultured for 10 days and then analyzed for gene expression of (A) Fshr, (B) Cyp19a1, and (C) Vegf. CTRL group, base media plus letrozole vehicle (dimethyl sulfoxide, DMSO); LET 30 group, CTRL media plus 30 ng/mL letrozole and DMSO; and LET 100 group, CTRL media plus 100 ng/mL letrozole and DMSO. Data are expressed as mean ± standard errors and have been log2 transformed. LET 30 and LET100 groups were compared with the control. *P < 0.05, by one-way ANOVA and Student–Newman–Keuls post-hoc analysis